FEBS PLC2016 preliminary program

	FEBS Lectures 9:15-13:00	FEBS Lab Exercises 14:30-18:30	Evening events 20:00
Monday June 27	10:00-14:00 Registration 13:00-14:00 Lunch 14:00-14:30 Welcome and Course remarks [ Ivana Kutá Smatanová & Pavlína Řezáčová] 14:30-14:45 Prologue by the rector of the USB [Libor Grubhoffer] 15:15-15:30 Lecture by Chair of the FEBS ACC [Beata G. Vértessy] 15:30-16:15 Principles of protein crystallization: The nature of Protein Crystals and the Physical Chemistry of their formation [Bernhard Rupp] 16:15-17:00 Crystallization of membrane proteins in lipidic systems [Martin Caffrey] 17:00-17:45 An introduction to crystal morphology and crystal growth mechanisms [Juan M. García-Ruiz] 17:45-18:30 Conventional crystallization methods and their modifications [Jeroen Mesters]		Welcome party at representative quarters of the castle
Tuesday June 28	9:00-9:45 Principles of protein crystallization II: Methods, evaluation, and properties of ?real? crystals [Bernhard Rupp] 9:45-10:30 Capillary counterdiffusion technique for protein crystallization and screening [Juan M. Garcia-Ruiz] 10:45-11:30 Interpretation of the crystallization drop results [Terese Bergfors] 11:30-12:00 Seeding Strategies for "Random" Crystal Screening and Crystal Optimization [Stefan Kolek] 12:00-12:30 From protein expression and purification to protein crystallization [Radka Chaloupkova] 12:30-13:15 Crystal observation using UVEX [Rachel Macmaster]	14:30-18:30 Conventional techniques and their modifications, crystallization of own proteins [J. Mesters] ?The secret life of your crystallization drop?? [B. Rupp] Crystallization of membrane proteins in lipidic system [M. Caffrey] Observation of crystal growth / Seeding [T. Bergfors] Capillary protein crystallization using counter-diffusion techniques [J. Gavira]	Theory of X-ray diffraction [Jeroen Mesters] Discussion with speakers of the day + posters
Wednesday June 29 11:15 Coffee&tea time	9:00-9:45 Preparation of protein samples for crystallization experiments [Pavlína Řezáčová] 9:45-10:30 Protein as the main variable in crystallization [Ľubica Urbániková] 10:45-11:15 "What to do if everything has failed" [Terese Bergfors] 11:15-12:00 Publication of scientific results with emphasis on crystallization communications [Howard Einspahr] 12:00-13:00 A historical perspective on protein crystallization from 1840 to the present day [Richard Giegé]	14:30-18:30 Observation of crystal growth / Seeding [T. Bergfors] Capillary protein crystallization using counter-diffusion techniques [J. Gavira] "Random" Microseeding [S. Kolek] Publication of scientific results with emphasis on crystallization communications [H. Einspahr] Optional exercise: ?The secret life of your crystallization drop?? [B. Rupp] Optional exercise: Cystals observation [R. Macmaster]	Theory of X-ray diffraction [Jeroen Mesters] Discussion with speakers of the day + posters
Thursday June 30 11:15 Coffee&tea time	9:00-9:45 Crystallization and crystallographic analysis in a microfluidic chip [Claude Sauter] 9:45-10:30 Optimization of Cryptic Leads Derived from Trace Fluorescent Labeling Screening [Marc L. Pusey] 10:45-11:30 Tips and tricks for protein crystal manipulation and handling [José A. Gavira] 11:30-12:15 DLS measurements prior to crystallization experiments [Christian Betzel] 12:15-13:00 Some methods to maximize the probability of protein crystallization [Da-Chuan Yin]	Dynamic light scattering [K. Dierks, Ch. Betzel] Trace Fluorescent Labeling for Protein Crystallization Screening [M. Pusey] Crystallization under oil [L. Govada] Crystallization in microfluidic chips [C. Sauter] Optional exercise: Conventional techniques and crystallization of own proteins [J. Mesters, Ľ. Urbániková]	Round table discussion + student presentations
Friday July 1 10:45 Coffee&tea time	9:00-9:45 Unconventional crystallization strategies and techniques for screening and optimisation [Naomi E. Chayen] 9:45-10:30 Large volume crystal growth in restricted geometry for neutron crystallography[Joe Ng] 10:45-11:30 Optimisation of crystal growth for neutron crystallography [Monika Budayová-Spano] 11:30-12:15 Preparation of Micro- and Nano-Crystals for Free-Electron-Laser and Synchrotron Radiation Sources [Christian Betzel] 12:15-12:45 A rational approach to crystallising proteins and the impact of micro seed matrix seeding [Allan D?Arcy] 12:45-13:05 Screening the diffraction quality of protein crystals [Vernon Smith]	Dynamic light scattering [K. Dierks, Ch. Betzel] Trace Fluorescent Labeling for Protein Crystallization Screening [M. Pusey] Crystallization under oil [L. Govada] Crystallization in microfluidic chips [C. Sauter] Optional exercise: Conventional techniques and crystallization of own proteins [J. Mesters, Ľ. Urbániková] Optional exercise: How to perform diffraction experiment? [V.Smith]	Farewell dinner
Saturday July 2 10:45 Coffee&tea time	9:00-12:30 Crystal observation, testing, handling, mounting and cryocooling [J. Brynda, P. Pachl]	Departure

Note:
For crystallization experiments, typical recipes using commercial proteins (lysozyme, glucose isomerase, thaumatin, protease A, etc.) will be used. In addition, students can bring their own proteins and carry out crystallization trials on these during the course.
A poster section is planned in order to encourage participants to disscuss their own work.